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CONTENTS (Liter) | Gelatin Peptone | 20.0 g |
Magnesium Chloride | 1.4 g | |
Dipotassium Sulfate | 10.0 g | |
Cetrimide | 0.3 g | |
Agar | 13.6 g | |
Final pH = 7.2 ± 0.2 at 25°C |
Suspend 45.3 G of powder in 1 L of distilled or deionized water. Add 10 mL of Glycerol supplement (MB-G1821). Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Pour into petri dishes.
CN (Cetrimide) Agar is a medium used for the isolation and cultivation of Pseudomonas aeruginosa. Gelatin peptone provides nitrogen, carbon and nutrients. Magnesium chloride and dipotassium sulfate promote production of pigment. Cetrimide inhibits the growth of many microorganisms except Pseudomonas spp. Agar is the solidifying agent.
Inoculate the specimen using a sterile loop to the medium. Incubate at 30 - 35°C for 18 - 48 hours up to 72 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: slightly opalescent
Color: light amber
Incubation conditions: 30 - 35°C / 18 - 48 hours up to 72 hours
Microorganism | ATCC | Inoculum CFU | Growth | Characteristics |
Pseudomonas aeruginosa | 9027 | 50-100 | good | yellow-green colonies |
Escherichia coli | 8739 | ≥10³ | inhibited | - |
Enterococcus faecalis | 29212 | ≥10³ | inhibited | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. Lowbury and Collins (1955) J. Clin. Pathol. 8:47
2. King, Ward and Raney. 1954. J. LabClin. Med. 44:301.
3. Lowbury. 1951. J. Clin. Pathol. 4:66.
4. Brown and Lowbury. 1965. J. Clin. Pathol. 18:752
5. Kiska and Gilligan. 1999. In Murray, Baron, Pfaller, Tenover and Yolken (ed.), Manual of clinical microbiology, 7 th ed. American Society for Microbiology, Washington, D.C.
6. Refer to the KP, USP and EP.
Cat. No : MB-C2137 CN (Cetrimide) Agar | 500 G |
( 출처 : 기산바이오 )