*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Harlequin® Tryptone Bile Glucuronide Agar (TBX)
Specifications |
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Analyte |
E. coli | ||||
Brand |
NEOGEN Culture Media | ||||
Formula |
Enzymatic Digest of Casein |
20.0 g/L | |||
Bile Salts No. |
1.5 g/L | ||||
X-glucuronide |
0.075 g/L | ||||
Agar |
15.0 g/L | ||||
Grams per Liter |
36.50 | ||||
Liters per 500g |
14.00 | ||||
Mass |
500 g | ||||
Package Dimensions |
4.60 IN x 2.90 IN x 8.60 IN | ||||
Package Weight |
1.35 LB | ||||
Plates per 500g |
750.00 | ||||
Preparation |
1. Suspend 36.5 grams of the medium in 1 liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45-50°C.
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Previous Item Number |
Product was previously known as Acumedia Product: 7692 Harlequin Tryptone Bile Glucuronide Agar (TBX); and LabM Product: HAL003 Harlequin™ TBGA/TBX (Tryptone Bile Glucuronide Agar) |
Harlequin® Tryptone Bile Glucuronide Agar (TBX) is used for the enumeration of E. coli in food and animal feed and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone Bile Glucuronide Agar is recommended by ISO 16649-1:2001, ISO 16649-2:2001 and ISO 16649-3:2015 for enumeration of β-glucuronidase positive Escherichia coli in products intended for human consumption or for the feeding of animals. The medium contains tryptone as a source of nitrogen and amino acids, bile salts act as a selective against Gram-positive organisms and agar is the gelling agent. 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-glucuronide) is a chromogen for the detection of the β-D-glucuronidase enzyme which is present in most E. coli* but not other coliforms. The intracellular enzyme present in E. coli is able to break the bond between the substrate and the chromophore which dimerizes is the presence of oxygen to form an insoluble colored compound within the cell. The accumulation of the colored chromophore results in blue colonies on the agar. *96-97% of E. coli are β-D-glucuronidase positive. A notable exception is E. coli O157:H7