*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Selective medium used for the isolation and identification of faecal coliforms detection in water, wastewater, shellfish and foods.
*Eqaully use with MFDS (MB-E1063K) , QIA (MB-E1063Q) and NIER (MB-E1063N).
CONTENTS (Liter) | Peptone | 20.0 g |
Lactose | 5.0 g | |
Bile Salt Mixture | 1.5 g | |
Dipotassium Phosphate | 4.0 g | |
Monopotassium Phosphate | 1.5 g | |
Sodium Chloride | 5.0 g | |
Final pH = 6.9 ± 0.2 at 25°C. |
Suspend 37.0 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121 °C for 15 minutes. Cool to 45-50°C in water bath. Mix well. Dispense in final tubes. If necessary, dispense the medium before autocleave.
EC (Escherichia coli) Broth is a selective medium used for the isolation and identification of faecal coliforms detection in water, wastewater, shellfish and foods. Peptone provides nitrogen, carbon, vitamins and minerals. Lactose is a fermentable carbohydrate. Bile salt mixture is the selective agent against gram-positive bacteria. Dipotassium phosphate and monopotassium phosphate are buffering agents. Sodium chloride supplies essential electrolytes for transport and maintains osmotic balance.
Inoculate the specimen with stab using a sterile needle to the medium. Shake gently for spreading microorganism. Incubate at 44.5 ± 0.2 °C for 24 ± 2 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free flowing, homogeneous
Color: pink-beige
Prepared medium
Appearance : clear
Color: light amber
Incubation conditions: 44.5 ± 0.2 °C / 24 ± 2 hours
Microorganism | ATCC | Inoculum CFU | Growth | Gas |
Escherichia col | 25922 | 50-100 | good | + |
Enterococcus faecalis | 29212 | ≥10³ | inhibited | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C.
1. Eaton, A.D., L.S. Clesceri, and A.E. Greenberg (ed.).1995. Standard methods for the examination fo water and wastewater, 19th ed. American Public Health Association, Washington D.C.
2. Association of Officialn Analytical Chemists. 1995. Bacteriological analytical manual , 8th ed. AOAC International, Gaithersburg, MD.
3. Vanderzant, C., and D.F. Splittstoesser (ed.). 1992. Compendium of methods for the microbiological examination of food, 3rd ed. American Public Health Association, Washington D.C.
4. Refer to the MFDS, QIA and NIER.
Cat. No : MB-E1063 EC (Escherichia coli) Broth | 500 G |
( 출처 : 기산바이오 )