*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for total microbial count in milk and dairy products.
* Equally use with MFDS (MB-P1040K), QIA (MB-P1040Q) and NIER (MB-P1040N).
CONTENTS (Liter) | Tryptone | 5.0 g |
Yeast Extract | 2.5 g | |
Dextrose | 1.0 g | |
Agar | 15.0 g | |
Final pH = 7.0 ± 0.2 at 25°C |
Suspend 23.5 G of powder to 1 L of destilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Mix well. Dispense in petri dishes.
PCA (Plate Count Agar) is a medium for total count of aerobic bacteria and heterotrophic facultative anaerobes in waters, liquids, foodstuff, milk, dairy products and in all human utilization products prepared according to the formulation. Tryptone and yeast extract provide nitrogen, carbon, vitamins and minerals. Dextrose is an energy source. Agar is solidifying agent.
Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 35 - 37°C for 24 - 48 hours or 72 ± 3 hours (QIA at 35 ± 1 °C for 48 ± 2 hours or at 30 ± 1 °C for 72 hours). Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: slightly opalescent, no precipitate
Color: light amber
Incubation conditions: 35 - 37 °C / 24 - 48 hours or 72 ± 3 hours
(QIA at 35 ± 1 °C for 48 ± 2 hours or at 30 ± 1 °C for 72 hours)
Microorganism | ATCC | Inoculum CFU | Growth |
Escherichia coli | 25922 | 50-100 | good |
Staphylococcus aureus | 25923 | 50-100 | good |
Bacillus subtilis | 9466 | 50-100 | inhibited |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C.
1. APHA (1985). Standard Methods for the Examination of Water and Wastewater, 16th edition.
2. ISO 4833: 2003. Microbiology- General Guidance for the enumeration of micro-organisms. Colony count technique at 30°C.
3. Standard Method 9215, 21th edition, Heterotrophic plate count.
4. HPA National Standard Method, W4, Colony count by the pour plate method.
5. Refer to the MFDS, QIA and NIER.
Cat. No : MB-P1040 PCA (Plate Count Agar) | 500 G |
( 출처 : 기산바이오 )