*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Selective medium used for the isolation of gram-negative enteric bacteria. *Equally use with MFDS (MB-E2165K) and QIA (MB-E2165Q).
CONTENTS (Liter) | Peptone | 10.0 g |
Lactose | 5.0 g | |
Sucrose | 5.0 g | |
Dipotassium Phosphate | 2.0 g | |
Eosin Y | 1.5 g | |
Sodium Chloride | 0.4 g | |
Methylene Blue | 0.065 g | |
Agar | 13.5 g | |
Final pH = 6.8 ± 0.2 at 25°C. |
Suspend 35.97 G of powder in 1L of distilled or deionized water. Heat to boiling until completely dissolve. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Mix well. Dispense in petri dishes.
EMB (Eosin Methylene Blue) Agar is a selective medium used for the isolation of gram-negative enteric bacteria. Peptone provides carbon, nitrogen, vitamins and minerals. Lactose is the fermentable carbohydrate. Sucrose is added to the medium as an alternative carbohydrate source for lactose-fermenting, gram-negative bacilli. Dipotassium phosphate is used as a buffering agent. Eosin-Y and methylene blue serve as differential indicators. Agar is the solidifying agent.
Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 36 ± 1°C for 24 ± 2 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: pink-purple
Prepared medium
Appearance : opalescent
Color: purple with a green-orange cast
Incubation conditions: 36 ± 1 °C / 24 ± 2 hours
Microorganism | ATCC | Inoculum CFU | Growth | Characteristics |
Enterococcus faecalis | 29212 | ≥10³ | partially inhibited | colorless colonies |
Escherichia coli | 25922 | 50 -100 | good | blue black colonies/ dark center/metallic sheen |
Salmonella typhimurium | 14028 | 50 -100 | good | colorless colonies |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration are evident. Store prepared medium at 2-8°C.
1. Levine, M.M., (1918). Differentiation of E. coli and B. aerogenes on a simplified Eosin-Methylene Blue Agar. J. Infect. Dis. 23:43.
2. Holt-Harris, J.E., and O. Teague. (1916). A new culture medium for isolation of Bacikllus typhosa from stools. J. Infect. Dis. 18:596-600.
3. Refer to the MFDS and the QIA.
Cat. No : MB-E2165 EMB (Eosin Methylene Blue) Agar | 500 G |
( 출처 : 기산바이오 )