*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Oxford Medium Base / Code: REF222530
Specifications | |||||
---|---|---|---|---|---|
Formula | Pancreatic Digest of Casein | 8.9g | |||
Proteose Peptone No. 3 | 4.4g | ||||
Yeast Extract | 4.4g | ||||
Tryptic Digest of Beef Heart | 2.7g | ||||
Starch | 0.9g | ||||
Sodium Chloride | 4.4g | ||||
Esculin | 1.0g | ||||
Ferric Ammonium Citrate | 0.5g | ||||
Lithium Chloride | 15.0g | ||||
Agar | 15.3g | ||||
Directions |
1. Suspend 57.5 g of the powder in 1 L of purified water. Mix thoroughly.
Difco™ or BBL™ Buffered Peptone Water – 20 g; Difco™ Buffered Peptone Casein Water – 20 g. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 10 minutes. Cool to 45-50°C. 4. To prepare Oxford Medium: Dissolve acriflavine (5.0 mg), cefotetan (2.0 mg), colistin sulfate (20.0 mg), cycloheximide (400.0 mg) and fosfomycin (10.0 mg) in 5 mL reagant grade ethanol and 5 mL purified water. Filter sterilize supplement before use. Add 10 mL of antimicrobic supplement to 1 L of molten Oxford Medium Base (45-50°C). Mix thoroughly. To prepare Modified Oxford Medium: Aseptically rehydrate one vial of Modified Oxford Antimicrobic Supplement with 10 mL of sterile purified water. Rotate in an end-over-end motion to dissolve the contents completely. Add 10 mL of rehydrated Modified Oxford Antimicrobic Supplement to 1 L of Oxford Medium Base (45-50°C). Mix thoroughly. 5. Test samples of the finished product for performance using stable, typical control cultures. | ||||
*Adjusted and/or supplemented as required to meet performance criteria. |
Oxford Medium Base is used to prepare Oxford Medium1 or Modified Oxford Medium2 for isolating and differentiating Listeria monocytogenes.