*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the isolation and identification of coliforms in water, foods, dairy and pharmaceutical products. *Equally use with MFDS (MB-L1202K), QIA(MB-L1202Q) and NIER (MB-L1202N).
CONTENTS (Liter) | Peptone | 5.0 g |
Beef Extract | 3.0 g | |
Lactose | 5.0 g | |
Final pH = 6.9 ± 0.2 at 25°C. |
Suspend 13.0 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Mix well. Dispense in tubes.
Lactose Broth is a medium used for the isolation and identification of coliforms in water, foods, dairy and pharmaceutical products. Peptone and beef extract provide nitrogen, carbon, vitamins and minerals. Lactose is a fermentable carbohydrate. Lactose fermenting bacteria produce gas.
Inoculate the specimen with stab using a sterile needle to the medium. Shake gently for spreading microorganism. Incubate at 36 ± 1°C for 18 - 48 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free flowing homogeneous
Color: light beige
Prepared medium
Appearance : clear
Color: light amber
Incubation conditions: 36 ± 1°C / 18 - 48 hours
Microorganism | ATCC | Inoculum CFU | Growth | Gas |
Escherichia coli | 25922 | 50-100 | good | + |
Klebsiella pneumoniae | 27736 | 50-100 | good | + |
Enterococcus faecalis | 29212 | 50-100 | good | - |
Salmonella typhimurium | 14028 | 50-100 | good | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C.
1. APHA (1972) Standard Methods for the Examination of Dairy Products, 13th Ed.
2. APHA (1985) Standard Methods for the Examination of Water and Washewater, 16th Ed.
3. Flowers, R.S., J. D’Aoust, W.H. Andrews, and J.S. Bailey.(1992). Salmonella, p. 371-442.
4. Refer to the MFDS, QIA and NIER.
Cat. No : MB-L1202 Lactose Broth | 500 G |
( 출처 : 기산바이오 )