*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti (ALOA)
Specifications | |||||
---|---|---|---|---|---|
Analyte | Listeria spp | ||||
Brand | NEOGEN Culture Media | ||||
Formula | Meat Peptone | 18.0 g/L | |||
Tryptone | 6.0 g/L | ||||
Yeast Extract | 10.0 g/L | ||||
Lithium Chloride | 10.0 g/L | ||||
Sodium Chloride | 5.0 g/L | ||||
Disodium Hydrogen Orthophosphate Anhydrous | 2.5 g/L | ||||
Sodium Pyruvate | 2.0 g/L | ||||
Glucose | 2.0 g/L | ||||
Glycerophosphate | 1.0 g/L | ||||
Magnesium Sulphate | 0.5 g/L | ||||
5-Bromo-4-Chloro-3Indolyl-β-d-Glucopyranoside | 0.05 g/L | ||||
Agar | 13.5 g/L | ||||
Grams per Liter | 70.50 | ||||
Litres per 500g | 7.00 | ||||
Plates per 500g | 390.00 | ||||
Package Dimensions | 2.76 IN x 2.76 IN x 3.74 IN | ||||
Package Weight | 0.31 LB | ||||
Preparation |
1. Suspend 70.5 g of the medium in 950 mL of purified water.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the medium. 3. Autoclave at 121°C for 15 minutes. 4. Cool to 45–50°C and add 2 vials of reconstituted NCM4002-0.5* supplement, each reconstituted using 5 mL of sterile deionized/RO water. Swirl to mix. 5. Add 2 vials of NCM4001-0.5* supplement (preheated to 48–50°C). Mix well with gentle end-overend mixing. | ||||
Previous Item Number | Product was previously known as LabM Product: HAL010 Harlequin® Listeria Chromogenic Agar |
Harlequin® Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended to diagnose diseases or other conditions in humans.
Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium's selectivity. Chromogenic activity is a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and a few strains of Enterococci and Bacilli.
The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. This enzyme activity will result in a halo of precipitation surrounding the target colonies.
Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).