*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the identification and determination of fluorescein production by Pseudomonas spp.
*Equally use with KFCC (MB-P1309C).
CONTENTS (Liter) | Casein Peptone | 10.0 g |
Meat Peptone | 10.0 g | |
Dipotassium Phosphate | 1.5 g | |
Magnesium Sulfate | 1.5 g | |
Agar | 15.0 g | |
Final pH = 7.2 ± 0.2 at 25°C |
Suspend 38.0 G of powder in 990 mL of distilled or deionized water. Add 10 mL of Glycerol supplement (MBG1821). Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Mix well. Pour into petri dishes.
Pseudomonas Agar F (Fluorescein) is a medium used for the identification and determination of fluorescein production by Pseudomonas spp. Peptones provide nitrogen, amino acids, and vitamins. Dipotassium phosphate is the buffering agent. Magnesium sulfate is a cofactor for many metabolic reactions. Agar is the solidifying agent. Glycerol acts as an energy source and enhances production of pigment.
Inoculate the specimen using a sterile loop to the medium. Incubate at 30 - 35°C for 24 - 72 hours. Read fluorescence under a long-wave UV light. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: slightly opalescent
Color: light amber
Incubation conditions: 30 - 35°C / 24 - 72 hours
Microorganism | ATCC | Inoculum CFU | Growth | Characteristics |
Pseudomonas aeruginosa | 27853 | 50-100 | good | greenish-yellow |
Pseudomonas aeruginosa | 9027 | 50-100 | good | greenish-yellow |
Escherichia col | 25922 | 50-100 | good | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. Blazevic, D.J., Koepcke, M.H., a. Matsen, J.M.: Incidence of identification of Pseudomonas fluorescens and Pseudomonas putida in the clinical laboratory. Appl.Microbiol., 25; 107-110 (1973).
2. Brodsky, M.H., a Nixon, M.C.:Rapid Method for detection of Pseudomonas aeruginosa on McConkey Agar under ultraviolet light. Appl.Microbiol., 26; 219-220 (1973).
3. DIN Deutsches Institut fur Normung e. V.: Deutsche Einheitsverfahren zur Wasser -, Abwasser und Schlammuntersuchung. Mikrobiologisches Verfahren (Gruppe K). Nachweis von Pseudomonas aeruginosa (K8). DIN 38411.
4. GEORGIA, F.R., a. POE, C.F.: Study of bacterial fluorescence in various media. I. Inorganic substances necessary for bacterial fluorescence. J. Bact., 22; 349 (1931).
5. GEORGIA, F.R., a. POE, C.F.: Study of bacterial fluorescence in various media. Ii . The production of fluorescence in media made from peptone. J.Bact., 23; 135 (1932).
6. KING, E.O., WARD, M.K. A RANEY, D.E.: Two simple media for the demonstration of pyocyanin and fluorescin. J. Lab.Clin.Med., 44; 401-307 (1954). Unites States Pharmacopoeia XXII, Chapter “Microbial limit Tests”, 1995.
7. Refer to the KFCC.
Cat. No : MB-P1309 Pseudomonas Agar F (Fluorescein) | 500 G |
( 출처 : 기산바이오 )