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*Equally use with MFDS (MB-T2280K) and QIA (MB-T2280Q).
CONTENTS (Liter) | Tryptose | 15.0 g |
Yeast Extract | 5.0 g | |
Soytone | 5.0 g | |
Ferric Ammonium Citrate | 1.0 g | |
Sodium Metabisulfite | 1.0 g | |
Agar | 20.0 g | |
Final pH = 7.6 ± 0.2 at 25°C |
Suspend 47.0 G of powder in 900 mL of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Aseptically add 10% of Egg Yolk Emulsion (MB-E1864) and 20 mL of D-Cycloserine solution (MB-C1810). Mix well. Pour into petri dishes.
1 vial contents | |
D-Cycloserine | 0.2 g |
*Alternatively use with MB-C1811Q. |
TSC (Tryptose-Sulfite-Cycloserine) Agar is a medium used for the isolation and cultivation of Clostridium perfringens. Tryptose, yeast extract and soytone provide nitrogen, carbon, vitamins and minerals. Ferric ammoni um citrate and sodium metabisulfite are indicators of sulfate reduction. D-Cycloserine inhibits the catalyst which are necessary for the biosynthesis of the bacterial cell wall.
Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 36 ± 1°C for 18 - 24 hours under anaerobic condition. Refer appropriate references for recommended test procedure
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: opalescent
Color: amber
Incubation conditions: 36 ± 1°C / 18 - 24 hours / anaerobic condition
Microorganism | ATCC | Inoculum CFU | Growth | Characteristics |
Clostridium perfringens | 13124 | 50-100 | good | black colonies |
Clostridium sporogenes | 11437 | 50-100 | good | black colonies |
Escherichia coli | 25922 | ≥10³ | inhibited | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
1. Sahidi S.A. and Ferguson A.R. (1971) Appl. Microbiol, 21 500-506. Harmon S.M., Kauttar D.A. and Peeler J.T. (1971) Appl.
2. Microbiol. 21 922-927. Hauschild A.H.W. and Hilsheimar R. (1973) Appl. Microbiol. 27. 78-82.
3. International standard ISO 7937 Microbiology of food and animal feeding stuffs-Horizontal method for enumeration of Clostridium perfringens - colony count technique.
4. Refer to the MFDS and QIA.
Cat. No : MB-T2280 TSC (Tryptose-Sulfite-Cycloserine) Agar | 500 G |
( 출처 : 기산바이오 )